Female NRG mice are superovulated with PMSG and hCG followed by oocyte collection. Sperm is collected from male NRG mice.
Panel (a,b) At 16–18 weeks post-infusion of human HSCs, DRAGA, DRAG, and A2 mice were examined for serum levels of human IgM (a) and human IgG (b) by ELISA.
Mice were examined for human B cell reconstitution by FACS using human CD19 Abs. Panel (a) shows the human B cell reconstitution rate (percentage of mice ...
Differential engraftment and kinetics of human lymphocytes in humanized female and male mice. 5-week-old NRG mice were irradiated with 450 cGy and i.v. ...
Efficient genetic manipulation of the NOD-Rag1-/-IL2RgammaC-null mouse by combining in vitro fertilization and CRISPR/Cas9 technology | Scientific Reports
Panel (a) splenic cells from DRAGA (n = 6), A2 (n = 3) and DRAG (n = 2) mice were stimulated for 36 h with PMA/ionomycin, and the cytokines secreted in cell ...
The history of the development on immunodeficient mice for humanized mice model. In retrospect, nude mice or SCID mice were the first immunodeficient ...
Panel (a) DRAGA and A2 mice (n = 3 per group, infused with HSC from the same donor) were immunized intramuscularly twice at two weeks apart with GIL peptide ...
Long term survival of transplanted NRG mice versus CD34+ cell dose. Newborn NRG mice were irradiated and injected intra-hepatically with the indicated dose ...
Engraftment versus CD34+ cell dose. Newborn NRG mice were irradiated and injected intra-hepatically with the indicated dose of purified CD34+ cord blood ...
FACS analysis of T cell subsets versus time after transplant. Newborn NRG mice were irradiated and injected intra-hepatically with 100,000 purified CD34+ ...
FACS analysis of CD8T cell subsets versus time after transplant. Newborn NRG mice were irradiated and injected intra-hepatically with the indicated dose of ...
FACS analysis of T cell reconstitution versus time after transplant. Newborn NRG mice were irradiated and injected intra-hepatically with 100,000 purified ...
Differential effect of HLA class-I versus class-II transgenes on human T and B cell reconstitution and function in NRG mice | Scientific Reports
Human viral infections in mice with reconstituted human immune system components. NOD-scid γcnull (NSG), NOD Rag1null γc−/− (NRG), BALB/c Rag2−/− γcnull ...
(a) PCR results of founder mice. Mice from two microinjection sessions were genotyped by PCR amplification and BamHI digestion.
Taking this a step further and engrafting these humanized NSG mice with human tumors are the next best thing to a human patient and are the future of ...
At 16–18 weeks post-infusion of HSCs, DRAGA, DRAG, and A2 mice were examined for the frequency of human CD4 T and CD8 T cells in spleen and thymus by FACS ...
NSG mice provide a powerful platform for engraftment of human cells and tissues. Limitations in the development and function of certain lineages of human ...
Differential effect of HLA class-I versus class-II transgenes on human T and B cell reconstitution and function in NRG mice | Scientific Reports
To establish Hu-PBL-SCID (human peripheral blood lymphocyte (PBL)-engrafted severe combined immunodeficiency (SCID)) mice, PBLs are delivered to adult ...
200 female and 200 male mice were obtained at weaning (birthdate +/- 3 days) from maximum barriers in both Bar Harbor and Sacramento facilities and weighed ...
NSG mice and recently developed NSG-based models can support engraftment with multiple types of human cells and tissues. These engrafted human cells support ...
CRISPR/Cas9-based disruption of type VII collagen by embryo injection. (a) Strategy using the CRISPR/Cas9 nuclease system to produce Col7a1−/− NSG mice .